123ArticleOnline Logo
Welcome to 123ArticleOnline.com!
ALL >> Health >> View Article

Rapid Strides In The Field Of Capillary Electrophoresis

Profile Picture
By Author: Scott eric
Total Articles: 8
Comment this article
Facebook ShareTwitter ShareGoogle+ ShareTwitter Share

High performance liquid chromatography (HPLC) has undergone a tremendous development in the past two decades.This growth has been possible because of the advent of high-performance packing materials, suitable instrumentation, and a thorough theoretical understanding of separations.

We are seeing a similar development in the field of capillary electrophoresis(CE).The development in CE of capillary pretreatment procedures and permanent coatings is similar to developments in chromatography decades ago.Research in CE—in its present form with fused-silica columns and on-column detection—is more than a decade old (Jorgenson and Lukacs, 1981, 1983), and commercial CE instruments have been available since 1988.

The field is moving rapidly, illustrated by the fact that already six annual symposia on CE have been held and a number of comprehensive textbooks (see, for example, the textbooks edited by Camillieri, 1993; Landers, 1994; and Guzman, 1993)have been published recently. The reviews by Karger (1993) and Landers et al. (1993) capture the state of the art of CE. A sampling of the type of proteinrelated applications ...
... for which CE has been utilized in analytical biochemistryand clinical/diagnostic assays is given below.

Purity Assays

• QC of a manufacturing process
• Quantitation of contaminants or excipients
• Screening of samples prior to protein sequencing

Structural Studies

• Peptide mapping
• Two-dimensional methods, i.e., HPLC-CE, IEC-CE
• Microheterogeneity of complex proteins e.g., glycoproteins,monoclonal antibodies, histones,transferrins
• Oligomerization

Binding Studies

• Calcium, zinc-binding proteins
• Antigen-antibody immune complexes
• Drug–protein complexes
• Protein–DNA complexes

Process Analysis

• Quantitation of the final product
• Enzymatic digestion monitoring
• Derivatization (labeling) monitoring
• Deamidation, disulfide bridge reduction
• Conformational changes

Stability Studies

• pH, environment, temperature effects

Mobility Measurements

• (Semi-)empirical models for prediction of separation

Clinical/Diagnostic

• Serum protein assays
• Hemoglobin variants; globin chains
• Neuropeptide isolation from brain tissue
• Apolipoproteins
• Cytokines, transferrins, metallothioneins
• Proteins in single cells
• Isoenzymes

As in HPLC, various separation modes are applicable to the analysis ofproteins and peptides: capillary zone electrophoresis(CZE), micellarelectrokineticcapillary chromatography (MECC), capillary isoelectric focusing(CIEF), capillary gel electrophoresis (CGE), and displacement electrophoresisor isotachophoresis (ITP).

While in CE, the separation of small peptides(in the CZE mode) often is relatively straightforward and well understood, itappears that no single strategy is applicable for large peptides and proteins.As might be expected, this is largely due to the wide diversity and complexityassociated with these biomolecules.

Thus, different strategies often work fordifferent protein separation problems, hence requiring different CE separationmodes.

Reference–https://www.beckmancoulter.com/wsrportal/bibliography?docname=727484-CEPrimer5.pdf

Total Views: 81Word Count: 372See All articles From Author

Add Comment

Health Articles

1. Hypertensive Cardiovascular Disease: Understanding The Connection Between High Blood Pressure And Heart Health
Author: Dr Siam Al Mobarak

2. Best Cataract Surgery In Badlapur: Your Guide To Optimal Eye Care
Author: Anil Eye Hospital

3. Achieve Your Dream Body With Brazilian Butt Lift, Liposuction, And Tummy Tuck Surgery In Chicago
Author: CIPlastic

4. Top Lifestyle Changes To Prevent Chronic Joint And Back Pain
Author: Adam Vital Hospital

5. Facelift For Different Age Groups: Best Options In Your 40s, 50s, And Beyond
Author: Dr.Sajan

6. Reduce Risk Of Heart Problems With Weight Loss
Author: Alexis Pelloe

7. Comprehensive Eye Examinations In San Antonio: Ensuring Optimal Vision Health
Author: Sharron Acosta

8. Major Breakthrough: Five-year Survival In Nsclc Patients Treated With First-line Pembrolizumab
Author: MD Newsline

9. Comprehensive Eye Examinations In Torrance: What To Expect
Author: East West Eye Institute

10. What Are Dental Implants Procedures ?
Author: Camas Periodontics

11. Why Cosmetic Dentistry Might Be Right For You? Feel Good To Smile And Be Confident In Every Facial Expression
Author: Vikram Vasisht

12. Wisdom Tooth Often Needs Extraction….because!
Author: Vikram Vasisht

13. How To Store Bulk Weed Properly To Maintain Freshness
Author: Jose Henry

14. Safeguarding Healthcare: Essential Cybersecurity Strategies For Protecting Patient Data
Author: Triyam Inc

15. Building A Foundation For Your Child’s Oral Health
Author: Ansley Colton

Login To Account
Login Email:
Password:
Forgot Password?
New User?
Sign Up Newsletter
Email Address: