Basic Info About Elisa Kits And Cytokines

By Author: Groshan Fabiola
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ELISA kits, where the term ELISA denotes Enzyme-Linked ImmunoSorbent Assay. It is also known by several other names such as, Enzyme Immunoassay or EIA etc. It is a biochemical technique used in immunology to detect the presence of an antibody or an antigen in a sample.

The ELISA kits are used as diagnostic tools in medicine and as well as plant pathology, as well as quality control check in different types of industries. ELISA kits are sought after because they are fast, affordable, can easily accommodate multiple samples, and does not really require highly specialized equipment.

Cytokines are small, secreted proteins which mediate as well as regulate immunity, inflammation, and hematopoiesis. Cytokines generally act at very low concentration over short distances and short time spans.

Cytokines are an incredible tool gifted by Mother Nature; their production and effectors functions are tightly regulated, and even the smallest amounts might have a huge impact. For your information one single cytokine may act on different cell types or exert different functions depending on the environment provided.

Cytokines ...
... are capable of stimulating target cells, which results in making additional cytokines; some act synergistically, while others act antagonistically. The network of cytokines acting in a particular situation or pathological condition can be extremely complex and inscrutable, For which broad analysis may be required instead of measurement of only a single one.

Coming back to Elisa Kits, while you are in the process of choosing ELISA kit, first and foremost it is advised that you know what your goals and purpose behind the ELISA kit is. This will help you determine which measurements would serve your purpose. For example if you want to go for ELISA Kits for measuring Cytokines production it is advised that before buying your equipment you have studied the functionality, usability and viability of the kit.

Before choosing Elisa kits for measuring Cytokines one should determine the optimal signal and lowest background for the ELISA against each other in a preliminary experiment. An appropriate range of serial dilutions for the cytokine standard should also be included. You will find suggested range on the Technical Data Sheet (TDS) for ELISA reagents. Handling instructions are lot-specific. For maximum recovery of cytokine, the vial of cytokine should be quick-spun before opening. Some key issues need to be considered while planning experiments that involve cytokine and chemokine protein measurements using ELISA kit.

Whether you re buying ELISA kits Protein or ELISA kits Cytokines, it is of great importance that you look for specification before buying your ELISA kit.

For more resources about ELISA kits or even about Cytokines please review this page http://www.biosupply.co.uk